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qiime2-amplicon
【效率与协作】Processes paired-end 16S amplicon reads into QIIME 2 ASVs and taxonomy with retained artifact provenance. Checks paired FASTQ manifests, primer orientation diagnostics, predicted post-trimming overlap, sample IDs, runtime versions and read retention, and guides selection of compatible taxonomic classifiers.
brand-kit-print-collateral
【设计与创意】Print collateral spec: item, finished and trim size, bleed, colour mode, stock and GSM, finish, safe margin, print method, quantity and unit cost. Use for cards and letterhead.
context-window-management
【效率与协作】Strategies for managing LLM context windows including summarization, trimming, routing, and avoiding context rot
context-window-management
【效率与协作】Strategies for managing LLM context windows including summarization, trimming, routing, and avoiding context rot
editing-agents-md
【文档与办公】Decide whether a rule belongs in an AGENTS.md / CLAUDE.md file, and write it so it holds. Use before adding, editing, or removing any instruction in a root or nested AGENTS.md, when a convention keeps getting broken and someone proposes documenting it, when reviewing a diff that touches one, or when a file has grown and needs a trim. Carries the enfo…
agent-transcript
【开发与工程】Requested GitHub PR/issue agent transcripts: redact, trim, preview, and insert safely.
tooluniverse-sequence-analysis
【数据与研究】Biological sequence analysis — gene/protein sequence retrieval (NCBI, Ensembl, UniProt), nucleotide/protein search, ortholog discovery, and FASTQ QC + alignment workflows (Trimmomatic, BWA, samtools, coverage depth). Use for sequence retrieval, sequence comparison, FASTQ QC analysis, and read alignment pre-processing.
tooluniverse-fastq-qc
【数据与研究】FASTQ quality control and adapter/quality-trimming decisions with local NGS tools — run FastQC on raw reads, summarize a project with MultiQC, interpret per-base sequence quality, per-base N content, adapter content, overrepresented sequences, sequence duplication and GC content, and decide whether (and how) to trim with fastp / Cutadapt before downs…
tooluniverse-sequence-analysis
【数据与研究】Biological sequence analysis — gene/protein sequence retrieval (NCBI, Ensembl, UniProt), nucleotide/protein search, ortholog discovery, and FASTQ QC + alignment workflows (Trimmomatic, BWA, samtools, coverage depth). Use for sequence retrieval, sequence comparison, FASTQ QC analysis, and read alignment pre-processing.
create-music-elevenlabs
【效率与协作】Generate an instrumental music bed via ElevenLabs Music, ROUTED THROUGH THE elevenlabs-proxy so it bills the Ads agent. Trims any sparse intro, loudnorm, fades the tail. Prompt + length from the template recipe. Use for the music layer of any video-ad format.
render-hand-swipe-flavor-listicle
【设计与创意】Render a 'hand-swipe flavor listicle' video from a config — the brand's REAL product cutouts each float on a flat flavor-matched color field under a persistent title and slide RIGHT-TO-LEFT one to the next like a phone-feed scroll, while a green-screen hand clip (back of hand toward camera, index finger up) is trimmed to its clean finger-up window, l…
render-multiworld
【设计与创意】Assemble a silent, music-led 3-world product-tour ad — trim and hard-cut-concat the per-world WIDE-arrival + top-down-macro clips, composite the HTML/Playwright brand end card (approved headline + handwritten product labels + arrows) over an AI flat-lay background, and mux one music bed into a 720x1280 web master. FREE, deterministic assembly (Playwr…
render-narrated-ugc-wardrobe-stitch
【设计与创意】Assemble a narrated-UGC "stitch reply" ad from a config — a single spoken VO carries a verbatim testimonial while ~30 per-cut i2v clips (one creator across ~5 wardrobes in ~3 worlds, plus product B-roll) are each trimmed to their EDL window built from the VO's Whisper word boundaries and hard-concatenated via filter_complex concat (never the demuxer,…
stitch-videos-ffmpeg
【效率与协作】Stitch video segments with ffmpeg concat, xfade, overlay, audio mux, and export settings, and trim a source into named clips at exact windows (trim_clips.py). Ships montage.py, a free montage assembler (python3 + ffmpeg, no keys). It takes a JSON EDL of clips and stills, normalizes them and hard-cuts them in order, burns captions from an SRT, a cue l…
aot-trimming
【数据与研究】Guidelines for making .NET libraries and applications trimming-safe and Native AOT compatible. Covers the trimming/AOT model, the MSBuild properties that enable analysis (IsTrimmable, IsAotCompatible, PublishTrimmed, PublishAot), the trimming attributes (RequiresUnreferencedCode, RequiresDynamicCode, DynamicallyAccessedMembers, UnconditionalSuppressM…
bio-alignment-amplicon-clipping
【效率与协作】Trim PCR primers from aligned reads in amplicon-panel BAMs using samtools ampliconclip. Use when processing SARS-CoV-2 ARTIC, hereditary cancer panels, ctDNA hot-spot panels, or any amplicon assay where primer-derived bases would falsely confirm reference at primer footprints.
bio-alignment-trimming
【数据与研究】Trim multiple sequence alignments using ClipKIT, trimAl, BMGE, Divvier, or HMMcleaner with mode selection guidance per downstream goal. Use when removing unreliable columns or contaminating residues before phylogenetic inference, HMM building, or selection analysis.
bio-chipseq-motif-analysis
【数据与研究】Discovers de novo motifs and tests known motif enrichment in ChIP-seq, ATAC-seq, or other peak sequences using HOMER, MEME-ChIP (STREME, CentriMo, TOMTOM, FIMO), monaLisa, and AME. Handles background selection (GC-matched, dinucleotide-shuffled, Markov order-2, peak-flanks), motif databases (JASPAR 2024 CORE PWMs, JASPAR 2026 deep-learning collection…