backend-patterns
backend-patterns
【效率与协作】后端架构模式、API设计、数据库优化以及适用于Node.js、Express和Next.js API路由的服务器端最佳实践。
THE SKILLS DIRECTORY
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botpress-automation
botpress-automation
【开发与工程】Automate Botpress tasks via Rube MCP (Composio). Always search tools first for current schemas.
cloudpress-automation
cloudpress-automation
【开发与工程】Automate Cloudpress tasks via Rube MCP (Composio). Always search tools first for current schemas.
compression
compression
【数据与研究】Use when auditing slow page loads, heavy assets, or rendering delays related to Enable text-based compression. Verify the actual bottleneck in DevTools, Lighthouse, or field data before recommending changes.
image-compression
image-compression
【开发与工程】Use when reviewing image assets, markup, and CDN or build transforms related to Compress images without quality loss. Check encoded size, rendered size, loading strategy, and above-the-fold impact together.
image-file-size
image-file-size
【效率与协作】Use when auditing the image pipeline, static assets in `public/`, CMS-managed media, or image CDN transforms. Separate file-size problems from dimension mismatches so you can tell whether the issue is compression, format choice, or responsive delivery.
public-relations
public-relations
【数据与研究】When the user wants help with public relations, earned media, press coverage, journalist outreach, or media strategy (not pull requests). Also use when the user mentions 'PR,' 'press,' 'press release,' 'media outreach,' 'pitch a journalist,' 'get featured,' 'media list,' 'media kit,' 'press kit,' 'newsjacking,' 'news hijack,' 'HARO,' 'Qwoted,' 'Featu…
adaptyv
adaptyv
【设计与创意】Uses the Adaptyv Bio Foundry API and Python SDK to design protein characterization experiments, estimate costs, submit sequences, monitor laboratory progress, and retrieve results. Applies to Adaptyv Foundry, its target catalog, binding screening and affinity assays, thermostability, expression, fluorescence, epitope binning, and enzyme activity work…
arboreto
arboreto
【数据与研究】Infers candidate gene regulatory networks from bulk or single-cell expression data using AertsLab Arboreto GRNBoost2 and GENIE3. Use for transcription factor-target association ranking, compatible Dask execution, sparse expression inputs, and network stability checks.
bulk-rnaseq
bulk-rnaseq
【数据与研究】Prepares bulk RNA-seq FASTQ, Salmon, STAR or featureCounts output for gene-level differential expression. Covers nf-core/rnaseq and standalone quantification, biological replication, strandedness, reference provenance, validated count assembly and a PyDESeq2 handoff. Use for FASTQ-to-counts analysis, nf-core/rnaseq configuration, STAR/Salmon quantifi…
cantera
cantera
【效率与协作】Runs Cantera homogeneous chemical reactors and evaluates ignition delay with mechanism provenance, conservation checks, and numerical refinement. Use for combustion kinetics, closed adiabatic ideal-gas constant-volume or constant-pressure ignition, temperature histories, or mechanism-specific ignition-delay comparisons.
cellxgene-census
cellxgene-census
【数据与研究】Queries the CZ CELLxGENE Census programmatically for versioned public single-cell and spatial transcriptomics data. Use when you need population-scale cell metadata, gene expression slices, Census summary counts, source H5AD URIs/downloads, embeddings, spatial Census data, or reference atlas comparisons across organisms, tissues, diseases, assays, an…
deepspot-m
deepspot-m
【效率与协作】Generates transcriptome-wide virtual spatial transcriptomics from H&E histology with DeepSpot-M. Used for predicted log1p-CPM expression from 224x224 tiles at about 20x, querying the released protein-coding gene panel by symbol, and whole-slide prediction after resolution-aware tiling with histolab.
genomic-intelligence
genomic-intelligence
【开发与工程】Predicts regulatory features, gene structure, and expression directly from DNA sequence using Genomic Intelligence's hosted transformer DNA language models — no local GPU or model weights. Six tasks over a REST API and a hosted MCP server (keyless public demo): promoter regions, splice donor/acceptor sites, enhancer activity, chromatin state, sequenc…
gget
gget
【数据与研究】Queries 20+ bioinformatics resources through CLI/Python. Supports quick lookups of gene info, BLAST/BLAT, viral sequence downloads, PDB/mmCIF structures, G2P residue annotations, enrichment analysis, OpenTargets, COSMIC, CELLxGENE, and 8cube mouse specificity/expression data. Best for interactive exploration and simple queries. For batch processing o…
ginkgo-cloud-lab
ginkgo-cloud-lab
【效率与协作】Guides protocol selection, input preparation, pricing checks, and browser ordering on Ginkgo Bioworks Cloud Lab (cloud.ginkgo.bio). Applies to cell-free, E. coli, and Pichia protein expression; HiBiT, A280, and LabChip readouts; IVT mRNA/circRNA synthesis; thermal shift assays; Echo-MS methods; SPR target onboarding; plate-reader assay onboarding; an…
marine-carbonate-chemistry
marine-carbonate-chemistry
【数据与研究】Solves seawater carbonate chemistry with PyCO2SYS for chemical oceanography, ocean acidification, and marine carbon-cycle research. Use for paired total alkalinity, dissolved inorganic carbon, pH, or seawater pCO2/fCO2 measurements; carbonate speciation; aragonite and calcite saturation; Revelle factors; lab-to-in-situ temperature and pressure correc…
pathway-enrichment
pathway-enrichment
【数据与研究】Performs pathway and gene-set enrichment analysis on gene lists or ranked gene data and interprets the results. Used when the user has a set of genes (differentially expressed genes from PyDESeq2/Scanpy, CRISPR-screen hits, cluster marker genes, proteomics hits) and wants to know which biological pathways, GO terms, or gene sets are over-represented …
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