bio-metagenomics-functional-profiling
bio-metagenomics-functional-profiling
Profiles the functional potential of shotgun metagenomes with HUMAnN 3's tiered search (MetaPhlAn prescreen, Bowtie2 pangenome, translated DIAMOND vs UniRef), giving gene-family (RPK) and MetaCyc pathway abundances stratified by species. Covers why a metagenome measures potential not activity, why dropping UNMAPPED/UNINTEGRATED biases everything, why strati…